Influence of Storage of Probiotic Broth Culture Filtrates on Their Antimicrobial Activity
DOI:
https://doi.org/10.15407/cryo27.04.311Keywords:
probiotics, Lactobacillus rhamnosus GG, Saccharomyces boulardii, metabolic products, antimicrobial activity, freezing, freeze-dryingAbstract
Nowadays there is no standard efficient technology for long-term storage of metabolite-containing products of microbial origin as well as no data about the advantages and expediency of using one or another method of storage for these products. Determining the optimal storage conditions will prevent the loss of biological activity of metabolites during storage both for scientific research and production. Metabolites were obtained by isolation from the producers by means of centrifugation and filtration after culturing probiotics in a liquid nutrient medium. We studied antimicrobial activity of filtrates of probiotic culture liquids with various inoculation doses right after obtaining and storage for 60 days in liquid, frozen and frozen-dried state at (4 ± 1)°C, (–23 ± 1)°C and under hypothermic conditions (4 ±1)°C, respectively. The storage of probiotic broth culture filtrates under the mentioned above conditions did not result in a significant reduction of their antimicrobial activity. The obtained results can be used when developing commerciol synbiotics.
Probl Cryobiol Cryomed 2017; 27(4): 311–321
References
Atlas R.M. Handbook of microbiological media. 4th ed. London New York: Boca Raton: CRC Press; 2010.
Basnakyan I.A. Cultivation of microorganisms with specified properties. Moscow: Meditsina; 1992.
Blynskaya E.V., Tishkov S.V., Alekseev K.V. Technological approaches to improving the process lyophilization of protein and peptide drugs. Russian Journal of Biotherapy 2017; 16(1): 6–11.
Bondarenko V.M. Metabolite probiotics: mechanisms of therapeutic effect in microecological disorders. Consilium Medicum 2005; (6): 437–443.
Buzoleva L.S., Somov G.P., Burtseva T.I. Comparative analysis of qualitative and quantitative composition of the amino and organic acids produced into cultural medium by Yersinia pseudotuberculosis at different temperatures. Journal of Microbiology, Epidemiology and Immunobiology 2006; (3): 12–16.
Chicherin I.Yu., Pogorelsky I.P., Lundovskikh I.A. Antibacterial activity and composition of supernatant of native culture Lactobacillus plantarum 8Р–Ð3. Zhurnal Mezhdunarodnoj Meditsiny 2013; (1): 131–139.
Cotter P., Ross R., Hill C. Bacteriocins – a viable alternative to antibiotics. Nat Rev Microbiol 2013; 11(2): 95–105. CrossRef PubMed
Courvalin P. Antibiotic resistance: the pros and cons of probiotics. Dig Liver Dis 2006; 38(2): 61–65. CrossRef
De Vuyst L, Leroy F. Bacteriocins from lactic acid bacteria: production, purification, and food applications. J Mol Microbiol Biotechnol 2007; 13(4): 194–199. CrossRef PubMed
Ermolenko E.I., Chernish A.Yu., Martsinkovskaya I.V. et al. Influence of probiotic enterococci on the growth of Streptococcus agalactiae. Journal of Microbiology, Epidemiology and Immunobiology 2007; (5): 73–77.
Ershova I.B., Gavrysh L.I., Kunegina E.N., Mochalova A.A. The significance of lactobacilli in the human body and the tactics of the correct choice of eubiotics. Novosti Meditsiny Farmatsii 2007; 17: 20–21.
Grischenko V.I., Gerodes A.G., Nikitchenko Yu.V. Effect of various methods of low temperature storage on antioxidative activity of human follicular fluid. Probl Cryobiol 1998; 24(2): 83–101.
Іsaenko O.Yu., Knysh O.V., Babych E.M. et. al. The influence of metabolic products of Lactobacillus rhamnosus GG on the test culture of staphylococci and corynebacteria. Bulletin of Problems Biology and Medicine 2017; 2(136): 246–251.
Isayenko O.Yu., Knysh O.V., Babych E.M. etc. Antimicrobial activity of metabolites of Saccharomyces boulardii against test cultures of Staphylococci and Corynebacteria. Pharmacology and Drug Toxicology 2017; 54 (3): 50–55.
Korotkykh O.O., Kalinichenko S.V. Evaluation of the exometabolites cytotoxic effect of Lactobacillus plantarum in vitro. Bulletin of Problems Biology and Medicine 2016; 2 (127): 158–162.
Lukoyanova O.L., Borovik T.E., Belyayeva I.A. et al. Influence of freezing and prolonged storage of expressed breast milk on its nutritive, biological values and microbiological safety. Voprosy Sovremennoi Pediatrii 2011; 10(1): 29–33.
Molokhova E.I., Sorokina Yu.V. Working out domestic metabolic probiotic and their standardization. Sibirsky Meditsinsky Journal (Tomsk) 2011; 26; 1(1): 29–33.
Nardid O.A. Effect of low temperatures on protein systems. Probl Cryobiol Cryomed 2014; 24(2): 83–101. CrossRef
Neschislyaev V.A., Stolbova M.G., Mokin P.A., Bondarev V.P. Development of probiotic capsule dosage form with immobilized bifidobacteria. Biopreparations Prevention Diagnosis Treatment 2013; 2(46): 35–38.
Neschislyaev V.A., Mokin P.A., Fedorova T.V. et. al. On the issue of development of highly effective metabolic probiotics. Actual areas (directions) of scientific research: from theory to practice 2016; 2: 15–17.
Neschislyayev V.A., Chistokhina L.P., inventors. Scientific and Production Association for Immunological Preparations "Microgen", assignee. Method of obtaining a biological stimulator. RU Patent 2001131538/13; 2004 Febr20.
Pikal M.J. Freeze–drying of proteins. In: Cleland J.L., Langer R, editors. Formulation and delivery of proteins and peptides. ACS Symposium Series 567; 1994. p. 120–133.
Ploskireva A.A., Gorelov A.V. Place of metabolic probiotics in the practice of a clinician. Russkij Meditsinskij Zhurnal 2014; 3: 232– 236.
Solovyeva I.V., Tochilina A.G., Novikova N.A. et. al. Study of biological properties of new Lactobacillus strains. Vestnik of Lobachevsky University of Nizhni Novgorod 2010; 2: 462–468.
Timokhina T.Kh., Markov A.Ð., Paromova Ya.I. et al. The way of receiving of bifidobacteria exometabolites with high antimicrobe activity. Meditsinskaya Nauka Obrazovaniye Urala 2016; 2: 152–154.
Ursova N.I. Topical and unsolved issues of probiotic therapy. Lechaschii Vrach 2013; 8: 60–65.
Vysekantsev I.P., Babinets O.M., Martsenyuk V.F. et. al. Correction of Bifidobacterium spp. and Lactobacillus spp. Populations in Mice with Experimental Intestinal Dysbiosis after Therapy with Enterosorbent-Immobilized Probiotics Stored at –80 and –196°C. Probl Cryobiol Cryomed 2015; 25(3): 267–286. CrossRef
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
