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Department for Low Temperature Preservation |
Research Fellows: |
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Department for Low Temperature Preservation is headed by Prof. Evgeny A. Gordienko, DSc (Biol). Major trend of the work performed was set to be fundamental theoretical studies of the causes and mechanisms of cryodamage at a cellular level. |
Gordiyenko O.I. Kholodnyy V.S. Kovalenko I.F. Kovalenko S.V. Kuleshova L.G. Makedonskaya V.A. Osetskiy A.I. Popivnenko L.I. Rozanov L.F. Reznikov V.I. Smolyaninova E.I. Todrin A.F. Vasilovskiy V.Yu. |
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Evgeniy A. Gordienko Prof., DSc (Biol) |
There has been developed and studied physico-mathematical theories and models of the phenomenon for transmembrane mass transfer and the processes occurring at various stages of the cycle of low temperature preservation [1], such as the inhibition phenomenon of the processes of activating type in high viscosity media, the phenomenon of lateral distribution of cell membrane components deformed by bending or isotropic tension, the phenomenon of hypertonic cryohemolysis, of crystallization cryohemolysis, the process of mechanical interaction of growing ice crystals with cells in the suspension being frozen. Basing on current notions about physical principles of cell activity there was defined for the first time and physically proved the basic theoretical model for endocytosis which described the general regularities for different types of the phenomenon [2, 3]. There has been theoretically proved the mechanism for conjugation between scalar biochemical reactions occurring in cell cytoplasm in relaxation-like auto-oscillations and a vector process of extracellular substances transfer inside cells. Proceeding from the endocytosis model built there have been explained and viewed some regularities of this phenomenon. We obtained the explanation for the entropy source in a multi-component solution taking into account the movement equations for its certain components and got the ratios between transport characteristics |
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of cell membranes and friction coefficients between some components for a motile membrane of a random shape [4, 5] Theoretical studies are combined with the experiments on a visual observation of freeze-thawing processes of cell suspensions using special devices to a light microscope. At the department there has been elaborated a unique cryomicroscopic complex for a visual study within a wide temperature range of the kinetics of physical and chemical processes, accompanying freeze-thawing of biological objects, kinetics of cell osmotic behavior at various cryorpeservation stages, cell permeability for cryoprotectants and water [6-9]. We perform the investigations on evaluating the mode of erythrocytes' membrane shift [10], peculiarities of erythrocytes transformation in different media [11-13], dilatometric studying of phase-structural transformations in cryoprotective media, cell suspensions and tissues within the range of low temperatures [14]. There has also been elaborated the experimental method for a quick determination of the coefficients for erythrocytes membrane permeability for electrically neutral substances [15-16] and erythrocytes distribution in the population on sphericity index [17], based on measuring the intensity of scattered by cells light. There was proved diagnostic efficacy of cell parameters studied for differentiation of normal and pathological states in blood, in particular, at hyper- and hypothyrosis, as well as at Diabetes mellitus [18, 19]. There have been studied the mechanisms for erythrocytes membrane permeability for the series of substances and the regularities were established for functioning of parallel ways of their penetration: through lipid bilayer and via protein channels of a certain size. There are determined the factors influencing the molecule permeability by that of another way [20-22].  |
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23, Pereyaslavskaya str., Kharkov, Ukraine 61015 Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine Tel: +380 57 373 4143, 373 3807, 373 3039; Fax: +380 57 373 3084 E-mail: cryo@online.kharkov.ua |
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